Publication details
Detekce Borrelia burgdorferi sensu lato v živočišných tkáních
| Title in English | Detection of Borrelia burgdorferi sensu lato in animal tissues |
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| Authors | |
| Year of publication | 2025 |
| Type | Conference abstract |
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| Description | The bacterium Borrelia burgdorferi sensu lato is the causative agent of a serious disease known as Lyme borreliosis. This bacterium has a wide range of potential sources—from various types of vectors to reservoir hosts. Borrelia burgdorferi sensu lato can be isolated from different organs as well as from body fluids of carriers or hosts. The aim of this work is the cultivation of Borrelia burgdorferi sensu lato from biological samples of animal tissues, subsequent DNA isolation from cultured media, and detection by PCR. Sequencing analysis is further used to determine the representation of individual strains of Borrelia burgdorferi sensu lato. The biological material originates from small mammals, ticks, and mosquitoes, which were captured in the field and processed in the laboratory for cultivation purposes. The culture media were regularly monitored using dark-field microscopy and subsequently preserved by freezing. DNA isolation and PCR analysis confirmed the presence of pathogenic Borrelia burgdorferi sensu lato. At present, collection and processing of additional samples is ongoing, which will also be included in this study. For this reason, final conclusions cannot yet be formulated. Nevertheless, in part of the samples, cultivation was successful and the presence of spirochetes was clearly demonstrated by microscopy. The presented contribution will provide an overview of the methodology and the results obtained. |