Publication details
Analýza mikrobiálních konsorcií vybraných přírodních pramenů se zaměřením na striktně anaerobní mikroorganismy
| Title in English | Analysis of Microbial Consortia of Selected Natural Springs with a Focus on Strictly Anaerobic Microorganisms |
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| Year of publication | 2026 |
| Type | Appeared in Conference without Proceedings |
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| Description | Natural mineral springs represent unique discharges of groundwater which, due to their specific chemical composition and the presence of gases (CO2, H2S), create extreme anoxic environments. These habitats host specific microbial communities with a high proportion of yet-to-be-cultivated organisms, often phylogenetically related to deep-sea lineages. The aim of this study was to characterize the structure of microbial communities at selected sites and to attempt targeted laboratory cultivation of key anaerobic groups. Within the methodology, microbial communities from sediments were analyzed using PCR, next-generation sequencing (NGS), and HCR-FISH. The samples were also used to establish cultivation experiments in specific media. The results indicate that the geochemical parameters of the sites, particularly the availability of sulfates and deep-origin gases, fundamentally determine community composition. Across the sites, variable abundances of groups such as Methanosarcinia, Hadesarchaeota, and Thermoplasmata were recorded. A strong correlation between the occurrence of lineages with reduced genomes (Promethearchaeia and Bathyarchaeia) and their bacterial partners (class Chloroflexia or sulfate-reducing bacteria) confirms their dependence on obligate syntrophy and interspecies hydrogen transfer. Within the cultivation part, representatives of Promethearchaeia, sulfate-reducing bacteria, and methanogenic archaea were successfully cultivated, representing a key step toward understanding their physiology and ecological roles in anoxic sediments. |
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