Publication details

Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments

Authors

PALEČEK Emil BRÁZDOVÁ Marie ČERNOCKÁ Hana VLK Daniel BRÁZDA Václav VOJTĚŠEK Bořivoj

Year of publication 1999
Type Monograph
MU Faculty or unit

Faculty of Science

Citation
Description Recently we have shown that wild-type human p53 protein binds preferentially to supercoiled (sc) DNA in vitro in both the presence and absence of the p53 consensus sequence (p53CON). This binding produces a ladder of retarded bands on an agarose gel. The intensity and the number of these hands are decreased by physiological concentrations of zinc ions. At higher zinc concentrations, binding of p53 to scDNA is completely inhibited.

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