Publication details
DNaseI immobilized on magnetic hydrogel particles in DNA analysis
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Proceedings |
| Conference | 20th International Symposium on the Separation and Analysis of Proteins, Peptides and Polynucleotides |
| MU Faculty or unit | |
| Citation | |
| Field | Biotechnology |
| Description | A simple separation of enzymes from reaction mixture can be achieved using enzymes immobilized on magnetic carriers. The aim of our work was to utilize enzymes immobilized on magnetic carriers for isolation and purification of chromosomal and plasmid DNAs. Properties of DNase I immobilized on both magnetic bead cellulose and poly(2-hydroxyethyl methacrylate)microspheres were studied. Activities of free and immobilized DNase I were studied in dependence on pH and the presence of activating bivalent cations. Moreover, biological activity of DNase I immobilized on both magnetic supports was tested by cleavage of plasmid pUC19 DNA. |
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