Project information
Dispersion polymerization of hydrofilic monomers for the preparation of magnetic carriers used in biotechnological application
- Project Identification
- GA203/00/1339
- Project Period
- 1/2000 - 1/2002
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- doc. Ing. Bohuslav Rittich, CSc.
- Cooperating Organization
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Institute of Macromolecular Chemistry of the ASCR, v. v. i.
- Responsible person Ing. Daniel Horák, CSc.
Project is aimed at the design of new hydrophilic magnetic carriers of spherical shape and uniform size in range of micrometers, which can be used in biotechnological applications. The carriers will be prepared by dispersion polymerization in the presenc e of various magnetic iron oxides. The effect of type and amount of iron oxide added, solvent mixture, monomer concentration, type and concentration of stabilizer and initiator and temperature on the properties of the resulting particles will be determin ed and the polymerization mechanism elucidated. Various affinity ligands, such as enzymes, antibodies, or reactive dyes will be bound to modified magnetic particles. For example, RNase, proteinase, lysozyme, or antibodies against some pathogenic bacteria will be immobilized on the magnetic particles. Activity of immobilized proteins will be tested in the preparation of crude cell lysate, or by means of immunomagnetic separation. Particles with immobilized bacteria will be used in polymerase chain reacti
Publications
Total number of publications: 18
2003
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Effect of reaction parameters on properties of dispersion-polymerized hydrophilic microspheres as supports for immobilization of proteins
Progres of Colloid Polymer Science, year: 2003, volume: 124, edition: 1
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Immunomagnetic separation of Salmonella cells using newly designed carriers
Journal of Chromatography A, year: 2003, volume: 1009, edition: 1-2
2002
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Effect of reaction parameters on properties of dispersion-polymerized hydrophilic microspheres as supports for immobilization of proteins.
Polymer Colloids: Preparation and Properties of Aqueous Polymer Dispersions, year: 2002
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Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles
Journal of Chromatography B, year: 2002, volume: 2002, edition: 774
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Chromatographic purification and separation of plasmid and lambda phage DNAs
22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides, year: 2002
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Identifikace terénních kmenů bakterií rodu Salmonella metodou PCR v přítomnosti intracelulárních inhibitorů PCR
VI. Pracovní setkání biochemiků a molekulárních biologů, year: 2002
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Immunomagnetic separation of Salmonella cells using newly designed magnetic carriers
22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides, year: 2002
2001
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Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods
The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, year: 2001
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Identifikace patogenů potravinového řetězce v přítomnosti inhibitorů PCR
V. Pracovní setkání biochemiků a molekulárních biologů, year: 2001
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Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles
The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, year: 2001