Publication details

Průkaz neutralizačních protilátek proti fosfolipáze D Arcanobacterium haemolyticum u nemocných s akutní tonzilitidou.

Title in English Detection of neutralizing antibodies against phospholipase D of Arcanobacterium haemolyticum in patients with acute tonsillitis.
Authors

VOTAVA Miroslav SKALKA Boris WOZNICOVÁ Vladana RŮŽIČKA Filip ZAHRADNÍČEK Ondřej ONDROVČÍK Petr KLAPAČOVÁ Lenka

Year of publication 2001
Type Article in Periodical
Magazine / Source Epidemiologie, mikrobiologie, imunologie
MU Faculty or unit

Faculty of Medicine

Citation
Field Microbiology, virology
Keywords Arcanobacterium haemolyticum - antibodies - neutralization test - phospholipase D - equi factor - acute tonsillitis
Description Neutralization test carried on a non-nutrient bloo agar prepared from sheep erythrocytes sensibilized with equi factor of Rhoococcus equi was used for the detection of antibodies against phospholipase D (PLD) of Arcanobacterium haemolyticum. Altogether, 433 sera from 404 patients aged 1 to 25 years with the symptoms of acute tonsillitis were examined. Antibodies against PLD were foun in 28 patients (6.9 %). In 116 sera from persons without the signs of tonsillitis, the antibodies against PL were detected in one case only (0.9 %). The titres reached the values from 4 to 64. Out of 84 paire sera, the significant change of titre, i.e. at least the four-fold one, was observed five times. The antiboies were found most often in children aged 11 to 15 years, 10 times out of 97 (10.3 %). In 253 patients with the tonsillitis, the result of the paralell bacteriologic examination of throat swabs was at the disposal. A. haemolyticum was successfully isolated in 3 cases, but only owing to the inhibition of staphylococcal hemolysis around its colonies. All three cases were also serologically positive. Consequently, our results show that neutralizing antibodies against PLD of A. haemolyticum can be detected in patients with tonsillitis.

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