Publication details
Analýza mutací v transgenu Drosophila melanogaster pomocí teplotní gradientové elektroforézy (TGGE) a sekvencování.
| Title in English | Analysis of mutations in Drosophila melanogaster transgene by temperature gradient gel electrophoresis (TGGE) and sequencing. |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | 24. Pracovní dny české a slovenské společnosti pro mutagenezu zevním prostředím |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Transgenic Drosophila melanogaster; TGGE; Sequencing |
| Description | Temperature gradient gel electrophoresis (TGGE) is a powerful electrophoresis methods for separation of nucleic acids. The aim of this work was to modify experimental parameters of TGGE to detect unknown mutations in fragment of lacZ transgene in Drosophila melanogaster. Approximately 500 bp long unspecific product was proven in one sample. Some of samples were sequenced. |
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