Publication details

Analýza mutací v transgenu Drosophila melanogaster pomocí teplotní gradientové elektroforézy (TGGE) a sekvencování.

Title in English Analysis of mutations in Drosophila melanogaster transgene by temperature gradient gel electrophoresis (TGGE) and sequencing.


Year of publication 2001
Type Article in Proceedings
Conference 24. Pracovní dny české a slovenské společnosti pro mutagenezu zevním prostředím
MU Faculty or unit

Faculty of Science

Field Genetics and molecular biology
Keywords Transgenic Drosophila melanogaster; TGGE; Sequencing
Description Temperature gradient gel electrophoresis (TGGE) is a powerful electrophoresis methods for separation of nucleic acids. The aim of this work was to modify experimental parameters of TGGE to detect unknown mutations in fragment of lacZ transgene in Drosophila melanogaster. Approximately 500 bp long unspecific product was proven in one sample. Some of samples were sequenced.
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