The influence of metal ions on the activity of immobilized nucleases

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; RUDOLF Ivo; HORÁK Daniel; LENFELD Jiří; BENEŠ Milan J.
• Year of publication: 2001
• Type: Article in Proceedings
• Conference: Life Sciences 2001
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: magnetic particles; DNaseI; metal ions; repeated use
• Description: DNaseI immobilized on hydrophilic supports - magnetic cellulose and magnetic poly(HEMA-co-EDMA) particles was prepared. Repeated use of immobilized DNase I activated by different metal ions on hydrolysis of chromosomal and plasmid DNAs was studied.

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