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Analýza fenolických kyselin artyčoku pomocí vysokoúčinné kapalinové chromatografie s coulometrickou detekcí

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Title in English Analytical procedure for determination of phenolic acids in artichoke (Cynara spp.) using HPLC with coulometric detection
Authors

SLANINA Jiří TÁBORSKÁ Eva MUSIL Pavel

Year of publication 2001
Type Article in Proceedings
Conference Biologicky aktivní fenolické látky v zemědělských plodinách
MU Faculty or unit

Faculty of Medicine

Citation
Field Analytic chemistry
Keywords Cynara; HPLC; phenolic acids; electrochemical detection
Description Analysis of artichoke phenolic acids using reversed-phase high-performance liquid chromatography with coulometric detection is described. The HPLC system consisted of an LiChrospher 100 RP-18 column (5 um, 125 x 4 mm) and a mobile phase with 35% methanol in 50 mM phosphate buffer (pH 2.5). High selectivity and a low detection limits were achieved at a low potential (0-150 mV). The method was applied to identification of caffeoylquinic acids metabolites in human urine after a single dose of an extract from the leaves of Cynara cardunculus taken orally.
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