Publication details
Western blot determination of IgG avidity in primary and secondary syphilis.
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Scripta medica (Brno) |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | low-avidity IgG; syphilis; Western blot |
| Description | IgG avidity to p15.5 kD, p17 kD, and p47 kD antigens of Treponema pallidum was analysed by a new avidity assay termed avidity Western blot. Group I consisted of sera from 27 patients with primary and secondary syphilis, group II comprised 10 subjects with latent syphilis. Low-avidity antibodies were directed against the p17 kD and p47 kD antigens in group I and against the p47 kD antigen in group II. No low-avidity IgG antibodies were detected by ELISA, a routine method for avidity assessment. |