Publication details

Discrimination of Armillaria species based on molecular biological methods and HPLC.



Year of publication 2002
Type Article in Proceedings
Conference Book of Abstracts of 7th Meeting of WG
MU Faculty or unit

Faculty of Science

Field Genetics and molecular biology
Keywords Armillaria; PCR; discrimination; HPLC
Description The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens). We have used the internal transcribed spacer (ITS), which is highly polymorphic and provides useful tool for taxonomic studies thanks to highly conserved sequences of these region. The resolution achieved on the HPLC system was comparable or better than that achieved by electrophoresis and HPLC has better performance in quantitation. Moreover, the restriction analysis of the isolates using HPLC allowed to clearly differentiate some heterozygots from homozygots.
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