Publication details

Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)



Year of publication 2003
Type Article in Proceedings
Conference Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003
MU Faculty or unit

Faculty of Science

Field Genetics and molecular biology
Keywords monogenea; diplozoids; PCR; RFLP; dignostic
Description The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites.
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