Publication details

Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Authors

SAPELNIKOVA Svetlana DOCK Eva SOLNÁ Renáta SKLÁDAL Petr RUZGAS Tautgirdas EMNEUS Jenny

Year of publication 2003
Type Article in Periodical
Magazine / Source Analytical and Bioanalytical Chemistry
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Screen-printed sensor; Cholinesterase; Enzyme electrode
Description The screen-printed technology of electrode fabrication allows construction of amperometric devices suitable for the combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor-arrays in the batch mode as well as in the flow-injection system are discussed.
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