Publication details
ZVÝŠENÁ EXPRESE VIMENTINU JAKO MARKER DIFERENCIACE MONOBLASTŮ BM2
| Title in English | INCREASE IN VIMENTIN EXPRESSION AS A MARKER OF BM2 MONOBLAST DIFFERENTIATION |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | VIII. Pracovní setkání biochemiků a molekulárních biologů. Sborník abstraktů. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | vimentin; monocyte/macrophage differentiation; marker; proteomics |
| Description | BM2 monoblasts can be induced to terminal differentiation using phorbol ester TPA. In this study we described changes of proteom of BM2 cells undergoing TPA-induced differentiation. Using protein gel electrophoresis we detected 55 kDa protein in maturing BM2 cells. This protein was identified as vimentin by mass spectrometry MALDI-TOF. This conclusion was confirmed by western blotting. The level of vimentin also increases in BM2 cells treated with trichostatin A and in RA-treated BM2 derivatives overexpressing retinoid acid receptors RAR or RXR. Increased level of vimentin can be thus used as differentiation marker in BM2 cells. |
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