Publication details
Srovnávací analýza genomu a proteomu polyvalentních stafylofágů
Title in English | Comparative genomics and proteomics of polyvalent staphylococcal bacteriophages |
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Authors | |
Year of publication | 2004 |
Type | Article in Periodical |
Magazine / Source | Bulletin Československé společnosti mikrobiologické |
MU Faculty or unit | |
Citation | |
Field | Genetics and molecular biology |
Keywords | bacteriophages; genomics; bioinformatics |
Description | Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGE with subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. |
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