Publication details

Srovnávací analýza genomu a proteomu polyvalentních stafylofágů

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Title in English Comparative genomics and proteomics of polyvalent staphylococcal bacteriophages
Authors

DOŠKAŘ Jiří PANTŮČEK Roman RŮŽIČKOVÁ Vladislava KAŠPÁREK Petr EYER Luděk KONEČNÁ Hana ZDRÁHAL Zbyněk PREISLER Jan

Year of publication 2004
Type Article in Periodical
Magazine / Source Bulletin Československé společnosti mikrobiologické
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords bacteriophages; genomics; bioinformatics
Description Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGE with subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry.
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