Publication details

Characterization of spirochetal isolates from arthrops collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis



Year of publication 2004
Type Article in Periodical
Magazine / Source Journal of Chromatography B
MU Faculty or unit

Faculty of Science

Field Microbiology, virology
Keywords Spirochetes;Arthropods;Fatty acid methyl esters
Description Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one "Spironema culicis" strain recovered from mosquito Culex pipiens and seven spirochetal strains (non identified) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography columm in conjuction with Microbial Identification System Sherlock (MIDI Inc.,Newark, DE,USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B.burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool preliminary identification of spirochetes isolated from ticks and dipterans.

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