Publication details
Ferrite supports for isolation of DNA from complex samples and polymerase chain reaction amplification
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Chromatography A |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Cobalt ferrite magnetic particles; DNA; PCR; dairy products |
| Description | The influence of cobalt ferrite particles, with non-modified or modified surface, on the PCR course was investigated. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of magnetic microspheres. This indirect method enabled verification of the suitability of designed particles for their application in PCR. Selected types of proposed magnetic particles were used for the isolation of PCR-ready bacterial DNA from various dairy products. Bifidobacterium and Lactobacillus DNAs were identified in dairy products using PCR. |
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