Publication details

Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor

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Authors

PACHERNÍK J. BRYJA V. EŠNER M. DVOŘÁK P. HAMPL Aleš

Year of publication 2005
Type Article in Periodical
Magazine / Source Physiol. Res.
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords cells
Description Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferating, survival and differentiation activities in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, III beta-tubulin, and GAP-43), up-regulation of early neural lineage-specific gene Mash-1, and down-regulation of early endoderm-specific genes alfa-fetoprotein and GATA-4. Moreover, LIF also slows growth and increases the level of apoptosis in differentiating cells.
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