Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
|Year of publication||2005|
|MU Faculty or unit|
|Description||Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed).|