Publication details
Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Collection Symposium Series, Biologically Active Peptides |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide |
| Description | Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system. |