Publication details
A new, sensitive method for enzyme kinetic studies of scarce glucosides.
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Amplex UltraRed reagent; beta-glucosidase; glucose assay; glucose-oxidase / peroxidase coupled reaction; phytohormone conjugation; zeatin-O-glucoside |
| Description | We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds. |
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