Publication details

Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection

Authors

MIKELOVÁ Radka HODEK P. HANUŠTIAK Pavel ADAM Vojtěch KŘÍŽKOVÁ Soňa HAVEL L. STIBOROVÁ Marie HORNA A. BEKLOVÁ Miroslava TRNKOVÁ Libuše KIZEK René

Year of publication 2007
Type Article in Periodical
Magazine / Source Acta Chim. Slov.
MU Faculty or unit

Faculty of Science

Citation
Field Plant production
Keywords electrochemical detection; liquid chromatography; isoflavones
Description Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.
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