Publication details

The testis-specific human protein RBMY recognizes RNA through a novel mode of interaction



Year of publication 2007
Type Article in Periodical
Magazine / Source EMBO REPORTS
MU Faculty or unit

Faculty of Science

Field Biochemistry
Keywords alternative splicing; NMR; protein-nucleic acid recognition; spermatogenesis; SELEX
Description The RBMY (RNA binding motif gene on Y chromosome) protein encoded by the human Y chromosome is important for normal sperm development. Although its precise molecular RNA targets are unknown at present, it is suggested that human RBMY (hRBMY) participates in splicing in the testis. Using systematic evolution of ligands by exponential enrichment, we found that RNA stem loops capped by a C(A/U)CAA pentaloop are high affinity binding targets for hRBMY. Subsequent nuclear magnetic resonance structural determination of the hRBMY RNA recognition motif (RRM) in complex with a high affinity target showed two distinct modes of RNA recognition. First, the RRM beta sheet surface binds to the RNA loop in a sequence specific fashion. Second, the beta2 beta3 loop of the hRBMY inserts into the major groove of the RNA stem. The first binding mode might be conserved in the paralogous protein heterogeneous nuclear RNP G, whereas the second mode of binding is found only in hRBMY. This structural difference could be at the origin of the function of RBMY in spermatogenesis.
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