Publication details

HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae

Authors

SUCHOMELOVÁ Jana TÁBORSKÁ Eva BOCHOŘÁKOVÁ Hana PAULOVÁ Hana MUSIL Pavel

Year of publication 2007
Type Article in Periodical
Magazine / Source Journal of Pharmaceutical and Biomedical Analysis
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-4N20703-5&_user=10&_coverDate=05%2F09%2F2007&_alid=594860707&_rdoc=1&_fmt=summary&_orig=search&_cdi=5266&_sort=d&_docanchor=&view=c&_ct=1&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&
Field Plant cultivation
Keywords Benzo[c]phenanthridine; Chelilutine; Chelirubine; Macarpine; Sanguirubine; Sanguilutine; Sanguinarine; Chelerythrine; RP-HPLC
Description The content of the seven quaternary benzophenanthridine alkaloids (QBA) sanguinarine (SA), chelerythrine (CHE), chelirubine (CHR), chelilutine (CHL), sanguilutine (SL), sanguirubine (SR) and macarpine (MA) was determined in the underground part of six plant species of the family Papaveraceae (Sanguinaria canadensis L., Dicranostigma lactucoides HOOK.f.et THOMS, Chelidonium majus L., Macleaya cordata (Willd.), Macleaya microcarpa (Maxim) and Stylophorum lasiocarpum (Oliv.)). HPLC method with reversed phase column Synergi Max RP C12 Phenomenex was used, mobile phase consisted of heptanesulfonic acid (0.01 mol/l) with triethanolamine (0.1 mol/l) in redistilled water, pH 2.5, acetonitrile gradient 25 60% during 25 min. Detection was performed at 280 nm. The highest content of SA and CHE was found in the roots of D. lactucoides (1.99%, resp. 3.43% of the dried roots). In rhizomes of S. canadensis was their content more then two times lower.
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