Publication details
Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Electroanalysis |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1002/elan.200603837 |
| Field | Electrochemistry |
| Keywords | streptavidin ; avidin ; modified electrode ; electrochemistry ; protein ; carbon paste electrode |
| Description | We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS). |
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