3. Structural and thermodynamical characterization of BclA, a lectin from Burkholderia cepacia complex (BCC)

• Title in English: Structural and thermodynamical characterization of BclA, a lectin from Burkholderia cepacia complex (BCC)
• Authors: LAMEIGNERE Emilie; MALINOVSKÁ Lenka; VARROT Annabelle; SLÁVIKOVÁ Margita; MITCHELL Edward P.; WIMMEROVÁ Michaela; IMBERTY Anne
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: 14th European Carbohydrate Symposium, EUROCARB 14
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: structure; lectin; pathogen; cepacia complex
• Description: Printed Glycan arrays performed by the Consortium for Functional Glycomics (CFG) demonstrated that BclA has a strict specificity for oligosaccharide with a terminal alpha-mannose residue. Thermodynamics of the interaction of BclA with methyl-mannoside and some other mannosylated oligosaccharides was studied by isothermal titration microcalorimetry (ITC). protein has been crystallized and the structure of the complex with the methyl-mannoside was solved at 1.7A resolution.

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