Multiplex panels of polymorphic microsatellite loci for two cryptic bat species of the genus Pipistrellus, developed by cross-species amplification within the family Vespertilionidae

• Authors: KAŇUCH Peter; FORNŮSKOVÁ Alena; BARTONIČKA Tomáš; BRYJA Josef
• Year of publication: 2007
• Type: Article in Periodical
• Magazine / Source: Molecular Ecology Notes
• MU Faculty or unit: Faculty of Science
• Field: Zoology
• Keywords: Chiroptera microsatellites pipistrellus pygmaeus sibling species
• Description: The paper presents multiplex panels of polymorphic microsatellites for two closely related cryptic species Pipistrellus pipistrellus and Pipistrellus pygmaeus. We tested the cross-species amplification of 34 microsatellite loci, originally developed for five vespertilionid bat species. Ten and nine polymorphic loci in P. pipistrellus (mean number of alleles per locus = 10.5) and P. pygmaeus (8.1), respectively, in three multiplex PCRs per species were amplified. All loci can be analysed in a single fragment analysis and can be used as markers to the study of evolution and the ecology of structured populations of socially living bats.

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