Publication details

Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.



Year of publication 2007
Type Article in Periodical
Magazine / Source European Biophysics Journal
MU Faculty or unit

Faculty of Medicine

Field Biophysics
Keywords amalgam electrodes; oligodeoxynucleotides detection
Description The application of a gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper ions is described. Our result shows that the sensitive detection of different ODNs can be also performed at the platinum amalgam-alloy electrode (PtAE) contrary to a low sensitivity at the silver amalgam-alloy electrode (AgAE).Electrochemical impedance spectroscopy (EIS), voltammetry, AFM, and DOE was used for the investigations of adsorption of oligonucleotides on the titanium surface and the effect of mechanical (polished, etched and/or gritted surface) and chemical modification of titanium surface was studied. From the EIS, voltammetric and optical measurements the conclusions of the effect of solvent and physical or chemical modification of titanium surface on the purine (AAG)12 and pyrimidine (TTC)12 oligonucleotide adsorption was delivered.
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