Publication details
Use of quantitative model to assess the fractional area of t-tubular membrane in ventricular cardiomyocyte
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Engineering Mechanics 2007 |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | cardiac cell; transverse-axial tubular system; tubular membrane area; quantitative modelling |
| Description | The transverse (t-) tubules of cardiac ventricular myocytes create a complex network of membrane invaginations. Many of the key proteins involved in excitation-contraction coupling appear to be located predominantly at the t-tubule membrane. Despite their importance, the fraction of cell membrane within the t-tubules remains unclear: for example, measurement of cell capacitance following detubulation in rat cardiomyocytes suggests 32%, whereas optical measurements suggest up to 65%. We have therefore investigated the factors that may account for this discrepancy. Calculation of the combinations of t-tubule radius, length and density that produce t-tubular membrane fractions of 32% or 56% suggest that the true fraction is at the upper end of this range. |
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