Publication details

Mechanical response of porous scaffolds for cartilage engineering

Authors

JANČÁŘ Josef SLOVIKOVA Alexandra AMLER Evžen KRUPA Petr KECOVÁ Helga PLÁNKA Ladislav GÁL Petr NEČAS Alois

Year of publication 2007
Type Article in Periodical
Magazine / Source Physiological Research
MU Faculty or unit

Faculty of Medicine

Citation
Field Traumatology and orthopaedic surgery
Keywords Chitosan; Nanofibers; Hyaluronic acid; Hydroxyapatite; Mesenchymal stem cells; Joint resurfacing
Description In this paper, the effect of modification of the cross-linked collagen I with hyaluronic acid, hydroxyapatite nanoparticles and chitosan nanofibers on the mechanical response of the compact scaffold material and the lyophilized porous scaffold construct has been investigated in the dry state at 37 oC under tensile loading. The results showed that the addition of hyaluronic acid reduced the tensile elastic modulus and slightly increased the ultimate deformation of crosslinked collagen I under the test conditions used. On the other hand, addition of hydroxyapatite nanoparticles and chitosan nanofibers, respectively, increased the elastic modulus of the modified collagen ten-fold and four-fold, respectively. The ultimate tensile deformation has been decreased by hydroxyapatite and increased by the chitosan nanofibers. It can be concluded that enhancing the stiffness of the compact scaffold material by adding rigid chitosan nanofibers can improve the resistance of the porous scaffolds against mechanical loading. SEM micrographs revealed the effect of the respective additives on the pore structure of the lyophilized collagen foams. lagen modified with chitosan nanofibers exhibited the most uniform pore structure. Moreover, chitosan nanofibers seemed to enhance mesenchymal cell growth compared to neat collagen I.
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