Publication details

Studium strukturálních změn membrán buněk HL-60 metodou freeze-etching

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Title in English Study of structural changes in membranes of HL-60 cells using freeze-etching method
Authors

VAŠKOVICOVÁ Naděžda ŠKORPÍKOVÁ Jiřina JANISCH Roman

Year of publication 2008
Type Article in Proceedings
Conference Sborník příspěvků XXXI. Dny lékařské biofyziky
MU Faculty or unit

Faculty of Medicine

Citation
Field Biophysics
Keywords plasma membrane; nuclear envelope; HL-60 cells; ultrasound; freeze-etching
Description Freeze-etching method was used to observe structure of membrane areas HL-60 cells. The attention was concentrated on the plasma membrane and nuclear envelope. Replicas of cells affected by ultrasound and handling conditions were observed, distribution of proteins in the area of plasma membrane and shape of the membrane and the distribution of nuclear pores in the nuclear envelope were studied. Cell respond to the mechanical stress affected by ultrasound is similar as a result of handling with cell culture i.e. local corrugation membrane surface.
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