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Optimalizace exprese a purifikace rekombinantního lektinu CV-IIL z patogenního mikroorganismu Chromobacterium violaceum
| Title in English | Optimization of expression and purification of recombinant lectin CV-IIL from pathogen Chromobacterium violaceum |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Študentská vedecká konferencia 23.apríl 2008, Zborník prispevkov, 1.zväzok, biologická sekcia |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | lectin; CV-IIL; Chromobacterium violaceum |
| Description | The protein engineering of the lectin CV-IIL from human opportunistic bacterium Chromobacterium violaceum - site directed mutagenesis. An optimization of CV-IIL ins HisTag protein expresion in E.coli different expresion systems and optimization purification with usage a metalloaffinity chromatography. |
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