Laser ablation process as the key point of GLIM

• Authors: HAVLIŠ Jan; NEJEZCHLEBOVÁ Hana; VACULOVIČ Tomáš; KANICKÝ Viktor
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: Book of abstracts XIXthe Slovak Czech Spectroscopic Conference
• MU Faculty or unit: Faculty of Science
• Field: Analytic chemistry
• Keywords: metalloprotein;speciation;gel electrophoresis;laser ablation;ICP MS
• Description: laser ablation has the key role in analysis of metalloproteins by means of GLIM. several parameters were optimised: gel form (drier dried, cellophane coating, frozen gel), laser power density and their dependence to particle number and size, and signal intensity. best parameters were found for gels frozen at -80 deg for 25 min.

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