Publication details

Oxytetracycline Assay in Pond Sediment

Authors

NEPEJCHALOVÁ L. SVOBODOVÁ Z. KOLÁŘOVÁ J. FRGALOVÁ K. VALOVÁ J. NÉMETHOVÁ Danka

Year of publication 2008
Type Article in Periodical
Magazine / Source Acta Veterinaria Brno
MU Faculty or unit

Faculty of Science

Citation
Field Water pollution and control
Keywords Antibiotics; HPLC; ELISA; medicated feeding stuff; fish
Description The fate of drug residues and their metabolites in the environment is relatively rarely investigated in the conditions of the Czech Republic, resulting in limited availability of scientific information. To demonstrate one example, we prepared a model study with medicated feedstuff containing oxytetracycline hydrochloride (OTC HCl), which was used in fish under normal conditions of use. The oxytetracycline (OTC) contents were determined in the sediments of the pond where the fish were treated. The ELISA method was used for OTC detection and the HPLC method was used for final quantification of OTC. The increasing contents of OTC in sediment depended on the repeated treatment and excretion of OTC by the fish.
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