Publication details
Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments
| Authors | |
|---|---|
| Year of publication | 1999 |
| Type | Article in Periodical |
| Magazine / Source | Oncogene |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | p53; DNA-p53 interaction; scDNA; divalent cation; gel electrophoresis |
| Description | Recently we have shown that wild-type human p53 protein binds preferentially to supercoiled (sc) DNA in vitro in both the presence and absence of the p53 consensus sequence (p53CON). This binding produces a ladder of retarded bands on an agarose gel. The intensity and the number of these hands are decreased by physiological concentrations of zinc ions. At higher zinc concentrations, binding of p53 to scDNA is completely inhibited. |