Publication details

New recording setup for ratiometric recording of action potentials by optical means

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Authors

BARDOŇOVÁ Jana PROVAZNÍK Ivo NOVÁKOVÁ Marie FIALOVÁ Kateřina SEKORA Jiří

Year of publication 2008
Type Article in Proceedings
Conference Computers in Cardiology
MU Faculty or unit

Faculty of Medicine

Citation
Field Physiology
Keywords ratiometric measurement;action potentials;optical method
Description Monophasic action potential (MAP) can be recorded from the heart surface by optical method via fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which a new ratiometric recording setup is used for recording MAPs from isolated rabbit hearts perfused according to Langendorff. MAPs recorded by new ratiometric recording setup are compared with simple optical MAP measurement without ratiometry.
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