Publication details

Structural and functional characterization of lectin AFL1 from Aspergillus fumigatus

Authors

KOMÁREK Jan KOSTLÁNOVÁ Nikola VARROT Annabelle IMBERTY Anne WIMMEROVÁ Michaela

Year of publication 2009
Type Article in Proceedings
Conference XIII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords lectin Aspergillus fumigatus
Description This study deals with structural and functional characterization of 34.6 kDa lectin AFL1 from ascomycete Aspergillus fumigatus. AFL1 was expressed in a recombinant form and purified by FPLC on mannose-agarose column. Binding properties of AFL1 were determined by SPR and ITC. Several promising crystals for X-ray diffraction have been obtained using hanging drop vapour diffusion technique combined with streak seeding technique.
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