Publication details

Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy

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Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Scanning electron microscopy (SEM) technique was used for research of body surface and surface structures in several taxa of parasites. The fixation methods and preparation of specimens from selected parasite species were optimised. The optimal fixation method was preservation in hot 4% formaldehyde and 3% glutaraldehyde that fixed the parasite body in a straight position and conserved surface structures with the exception of fine cilia. For conservation of ciliate structures the use of the same fixation but at room temperature is more suitable. From the class Monogenea were studied all developmental stages (egg, free-swimming invasive larva oncomiracidium, diporpa, juvenile and adult) of Eudiplozoon nipponicum (Goto, 1891), parasite from the gills of carp. Tegument and sensory structures (type, distribution and function) on body surface were compared among developmental stages. The use of the SEM methodology in small nematodes was found to be necessary for studies of cephalic structures and the caudal end in males, where the study of form and distribution of cloacal and caudal papillae by light microscopy produces inaccurate results. From the class Nematoda parasites from different hosts (from an invertebrate, fishes, a reptile, birds and orang-unan) were studied using SEM.
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