Publication details

Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.



Year of publication 2001
Type Article in Periodical
Magazine / Source Molecular and Biochemical Parasitology
MU Faculty or unit

Faculty of Science

Field Microbiology, virology
Keywords Trichomonads; RNA-dependent RNA polymerase; RNAP; amanitin
Description Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes.

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