Publication details

Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening

Authors

HÉGROVÁ Blanka FARKOVÁ Marta MACUCHOVÁ Simona HAVEL Josef PREISLER Jan

Year of publication 2009
Type Article in Periodical
Magazine / Source Journal of Separation Science
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords Antifungal peptides; Barley; Gushing; MALDI-TOF MS; SDS-PAGE
Description The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (|r|Ao0.07, 0.344), (|r|Ao0.01, 0.494), respectively, as detected by both methods.
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