Publication details

Separation of similar yeast strains by IEF techniques

Authors

HORKÁ Marie RŮŽIČKA Filip HOLÁ Veronika ŠLAIS Karel

Year of publication 2009
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Medicine

Citation
Field Analytic chemistry
Keywords IEF; kandida; biofilm; lýza; identifikace
Description The possibility to separate of the conidia of filamentous fungi, A. niger, A. fumigatus, P. chrysogenum, F. solani and A. flavus, by capillary electromigration techniques was verified in this study. The optimized protocols for their separation by CIEF and CZE with UV and fluorometric detection were developed. According to the preliminary experiments the isoelectric points of the conidia of different strains were not hosts specific. The isoelectric points of the native conidia and the conidia dynamically modified by PB-PEG are comparable. At the electromigration techniques with fluorometric detection down to ten conidia injected into the capillary and dynamically modified by the non-ionogenic tenside were detected
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