Simultaneous HPLC determination of tolbutamide, phenacetin and their metabolites as markers of cytochromes 1A2 and 2C6/11 in rat liver perfusate

• Authors: JUŘICA Jan; KONEČNÝ Jiří; ZAHRADNÍKOVÁ Lucia; TOMANDL Josef
• Year of publication: 2010
• Type: Article in Periodical
• Magazine / Source: Journal of Pharmaceutical and Biomedical Analysis
• MU Faculty or unit: Faculty of Medicine
• Web: http://dx.doi.org/10.1016/j.jpba.2010.01.028
• Field: Biochemistry
• Keywords: CYP2C6; CYP1A2; metabolic activity; marker
• Description: The new original method for assessment the metabolic activity of both rat CYP1A2 and CYP2C6/11 is presented in the paper. The method of simultaneous determination of phenacetin, tolbutamide and their metabolites is rapid simple, repeatable and precious and sensitive enough for pharmacokinetic studies.

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