Publication details

Physiological study of Antarctic green algal lichen photobionts: An optimized method of isolation and culturing technique



Year of publication 2009
Type Article in Proceedings
Conference Structure and Function of Antarctic Terrestrial Ecosystems. Book of Abstracts and Contributed Papers
MU Faculty or unit

Faculty of Science

Field Botany
Keywords isolation techniques; culturing; lichen symbiont
Description Photobiont cells can be more or less successfully isolated from all lichens. Knowledge of lichen anatomy offers important information for isolation of photobionts. In our laboratory, we have been dealing with algal partner cultivation for some 6 years. Here we bring an overview of the method used with few optimizing points. For photobiont isolation from lichen thalli, we optimize the techniques of differential centrifugation combined with subsequent filtration of cell suspension.
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