Publication details
Crystallization and initial X-ray diffraction studies of the flavoenzyme NAD(P)H:(acceptor) oxidoreductase (FerB) from the soil bacterium Paracoccus denitrificans
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Peer-reviewed scientific article |
| Magazine / Source | Acta crystallographica. Section F Structural biology and crystallization communications |
| MU Faculty or unit | |
| Citation | |
| web | http://www3.interscience.wiley.com/journal/123345936/abstract |
| Field | Biochemistry |
| Keywords | PSEUDOMONAS-PUTIDA; QUINONE REDUCTASE; FERRIC REDUCTASE; FLAVOPROTEINS; CRYSTALS; PROTEIN |
| Description | The flavin-dependent enzyme FerB from Paracoccus denitrificans reduces a broad range of compounds, including ferric complexes, chromate and most notably quinones, at the expense of the reduced nicotinamide adenine dinucleotide cofactors NADH or NADPH. Recombinant unmodified and SeMet-substituted FerB were crystallized under similar conditions by the hanging-drop vapour-diffusion method with microseeding using PEG 4000 as the precipitant. FerB crystallized in several different crystal forms, some of which diffracted to approximately 1.8 A resolution. Structure determination by the three-wavelength MAD/MRSAD method is now in progress. |
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