Publication details

Alterations in the vascular architecture of the dorsal root ganglia in a rat neuropathic pain model

Investor logo
Authors

KUBÍČEK Luboš KOPÁČIK Roman KLUSÁKOVÁ Ilona DUBOVÝ Petr

Year of publication 2010
Type Article in Periodical
Magazine / Source Annals of Anatomy
MU Faculty or unit

Faculty of Medicine

Citation
Field Neurology, neurosurgery, neurosciences
Keywords vascular architecture dorsal root gangion neuropathic pain model rat
Description An alteration in the structural arrangement of blood vessels identified by RECA immunohistochemistry was studied in a rat L4 dorsal root ganglia (L4-DRG) neuropathic pain model. We compared a threedimensional (3-D) reconstruction of the vascular architecture surrounding bodies of the primary sensory neurons in the L4-DRG of naive rats with that of rats that had surgically undergone unilateral sciatic nerve ligature. Rhodamine-conjugated dextran (Fluoro-Ruby) was used for retrograde labelling of neurons, the axons of which had been injured by nerve ligature. In contrast to DRG from naive rats and contralateral DRG from operated rats, an increased proportion of RECA+ vascular area and the appearance of nest-like arrangements of blood vessels around neuronal bodies with injured axons were observed in L4-DRG ipsilateral to the sciatic nerve ligature. Fractal analysis confirmed a higher degree of vascular branching, irregularity, and tortuosity in L4-DRG related with sciatic nerve injury. The results suggest that nerve injury induces changes in vascular architecture in associated DRG
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info