Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94

• Authors: PRUDNIKOVA Tanyana; CHALOUPKOVÁ Radka; SATO Yukari; NAGATA Yuji; DEGTJARIK O.; KUTÝ Michal; REZACOVA P.; DAMBORSKÝ Jiří; KUTA-SMATANOVÁ Smatanova I.
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Crystal Growth & Design
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1021/cg1013363
• Field: Biochemistry
• Keywords: Crystallization; X-ray;Haloalkane dehalogenases
• Description: The DbeA1 variant of a novel haloalkane dehalogenaseDbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.

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