Publication details

Amino Acids Footprinting Based on Capillary Electrophoresis for Non-invasive Assessment of Human Embryo Viability

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Authors

MÁDR Aleš FOLTOVÁ Kateřina ŽÁKOVÁ Jana LOUSOVÁ Eva CRHA Igor GLATZ Zdeněk

Year of publication 2011
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Nowadays an embryo viability assessment relies upon an embryo morphology evaluation under a light microscopy. However, implantation rates of embryos selected in this way in general do not exceed 35 %. In order to increase chances of a pregnancy, multiple embryos are transferred in spite of the fact that health issues related to a multiple pregnancy occur. A new millennium began with a request made by the world leading embryologists to find an alternative non-invasive selection method. Metabolomic profiling of a spent embryo culture medium seems to be an appropriate way and analytical tools such as HPLC-MS/MS, 1H-NMR, Raman and Near-infrared spectroscopy were already examined for this purpose. Suitability of a capillary electrophoresis for amino acids footprinting is presented, pitfalls revealed and discussed.
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