The effects of PAHs and N-PAHs on retinoid signalling and Oct-4 expression in vitro.

• Authors: BENÍŠEK Martin; KUBINCOVÁ Petra; BLÁHA Luděk; HILSCHEROVÁ Klára
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Toxicology Letters
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.toxlet.2010.11.011
• Field: Environment influence on health
• Keywords: retinoids; differentiation; Oct-4; PAHs; N-PAHs
• Description: This study investigated PAHs and N-PAHs interference with retinoid signaling in vitro by modulating all-trans retinoic acid (ATRA) mediated response in a reporter gene assay using P19/A15 cell line. Further, effects on pluripotency and differentiation processes were evaluated by measuring octamer-4 (Oct-4), an important pluripotency marker and master differentiation factor. Our results further extend the knowledge of PAH and N-PAH in vitro effects and indicate that these environmental toxicants may have influence on differentiation process and embryonic development by interfering with ATRA signaling and by modulating levels of Oct-4.

Related projects

• Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
• New molecular biology and biochemical methods to monitor estrogens and other endocrine disruptors in the environment of the C.R.
• Centrum pro výzkum toxických látek v prostředí