Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders

• Authors: JANEČKOVÁ Hana; HRON Karel; WOJTOWICZ Petr; HLÍDKOVÁ Eva; BAREŠOVÁ Anna; FRIEDECKÝ David; ŽÍDKOVÁ Lenka; HORNÍK Petr; BEHULOVÁ Darina; PROCHÁZKOVÁ Dagmar; VINOHRADSKÁ Hana; PEŠKOVÁ Karolína; BRUHEIM Per; SMOLKA Vratislav; ŠŤASTNÁ Sylvie; ADAM Tomáš
• Year of publication: 2012
• Type: Article in Periodical
• Magazine / Source: Journal of Chromatography A
• MU Faculty or unit: Faculty of Medicine
• Web: http://www.elsevier.com/locate/chroma
• Doi: http://dx.doi.org/10.1016/j.chroma.2011.09.074
• Field: Analytic chemistry
• Keywords: targed metabolomics inherited metabolic disorders tandem mass spectrometry hierarchical cluster analysis
• Description: Metabolomics has become an importatnt tool research and diagnosis of human diseases. In this work we focused on the diagnosis of inherited metabolic disorders (IMDS) in plama samlpes using a targed metabolomics approach. The plasma samples were analysed with the flow injections analysis method. All experiments were performed on a QTRAP 5500 tandem mass spectometer (AB SCIEX, USA)with electorospray ionization. This study has revealed that targed metabolomic tools with automated and unsupervised processing can be applied for the diagnosis of various IMDs.