Publication details

Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients

Authors

KONEČNÁ Hana MULLER Lukáš DOSOUDILOVÁ Hana POTĚŠIL David BURŠÍKOVÁ Jana ŠEDO Ondrej MÁROVÁ Ivana ZDRÁHAL Zbyněk

Year of publication 2012
Type Article in Periodical
Magazine / Source J. Agric. Food Chem.
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://pubs.acs.org/doi/abs/10.1021/jf204475e
Doi http://dx.doi.org/10.1021/jf204475e
Field Analytic chemistry
Keywords beer proteome; two-dimensional gel electrophoresis; OFFGEL prefractionation; mass spectrometry; Hordeum vulgare; Saccharomyces species
Attached files
Description Two-dimensional gel electrophoresis in combination with mass spectrometry has already been applied successfully to study beer proteome. Due to the abundance of protein Z in beer samples, prefractionation techniques might help to improve beer proteome coverage. Proteins from four lager beers of different origins were separated by two-dimensional electrophoresis (2-DE) followed by tandem mass spectrometric analysis. Initially 52 proteins mostly from Hordeum vulgare (22 proteins) and Saccharomyces species (25 proteins) were identified. Preparative isoelectric focusing by OFFGEL Fractionator was applied prior to 2-DE to improve its resolution power. As a result of this combined approach, a total of 70 beer proteins from Hordeum vulgare (30 proteins), from Saccharomyces species (31 proteins), and from other sources (9 proteins) were identified. Of these, 37 proteins have not been previously reported in beer samples.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info